Objective
- To run UV-vis and Fluorescence on 0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated) in various organic solvents.
Description
- 10.6mg of the solid (0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 7 phosphate buffer (evaporated)) was added to 1 mL of MeOH.
- 11.0mg of the solid was added to 1mL of Acetone
- 11.1mg of the solid was added to 1mL Acetonitrile
- 11.3mg of the solid was added to 1mL Ethyl Acetate
- 11.3mg of the solid was added to 1mL water
- 11.4mg of the solid was added to 1mL chloroform
- each solvent was used as a blank.
- The data was corrected for the blank and a corrected baseline.
- UV-vis scanned from 200-800nm
- Fluorescence was run with an emission scan of 310-500nm and excitation at 290nm. The emission and excitation slits were 10.0nm, and the scan speed (nm/min) was 100.
Data
- Fluorescence of solid in MeOH
- UV-vis of solid in Acetone
- Fluorescence of solid in Acetone
- UV-vis of solid in Acetonitrile
- Fluorescence of solid in Acetonitrile
- UV-vis of solid in Ethyl Acetate
- Fluorescence of solid in Ethyl Acetate
- Fluorescence of solid in Water
- UV-vis of solid in Chloroform
- Fluorescence of solid in Chloroform
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Project name
