Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23"

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(Experimental Protocol)
(Experimental Protocol)
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==Experimental Protocol==
 
==Experimental Protocol==
  
'''Preparing Phosphate Buffer'''
+
'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
 +
 
 +
*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
 +
**The pH of the final solution will be 7.4.
 +
**This buffer can be stored for up to 1 mo at 4°C.
 +
*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
  
 
'''Running ADA Kinetics'''
 
'''Running ADA Kinetics'''

Revision as of 12:15, 23 January 2013

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Objective

  • To complete project proposal
  • To complete experimental protocol for next week.

Project Proposal

Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

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