Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23"

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*To complete experimental protocol for next week.
 
*To complete experimental protocol for next week.
  
==Project Proposal==
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==Experimental Protocol==
 
==Experimental Protocol==
  
'''Preparing Phosphate Buffer'''
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'''Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)'''
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*Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
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**The pH of the final solution will be 7.4.
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**This buffer can be stored for up to 1 mo at 4°C.
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*Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)
  
 
'''Running ADA Kinetics'''
 
'''Running ADA Kinetics'''
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*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29| next Tuesday]].
 
*The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2013/01/29| next Tuesday]].
  
[[Image:ADA_kinetics_table.png]]
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[[Image:ADA_kinetics_table.png|px80]]
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*To prepare a 30 mM stock solution of adenosine:
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<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g}{L}</math> of adenosine in buffer
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*To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.
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Revision as of 12:24, 23 January 2013

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Objective

  • To complete project proposal
  • To complete experimental protocol for next week.


Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

px80

  • To prepare a 30 mM stock solution of adenosine:

Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{0.030mol}{L}} of adenosine × Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{267.24 g}{1 mol}} = Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{8.0172g}{L}} of adenosine in buffer

  • To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.