Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23"

From OpenWetWare
Jump to: navigation, search
(Experimental Protocol)
(Experimental Protocol)
Line 39: Line 39:
*To prepare a 30 mM stock solution of adenosine:  
*To prepare a 30 mM stock solution of adenosine:  
.030  <math>\frac{mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math>
<math>\frac{0.030mol}{L}</math> of adenosine × <math>\frac{267.24  g}{1  mol}</math> = <math>\frac{8.0172g adenosine}{L buffer}

Revision as of 13:20, 23 January 2013

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2013/01/23</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>


  • To complete project proposal
  • To complete experimental protocol for next week.

Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.


  • To prepare a 30 mM stock solution of adenosine:

[math]\frac{0.030mol}{L}[/math] of adenosine × [math]\frac{267.24 g}{1 mol}[/math] = <math>\frac{8.0172g adenosine}{L buffer}