Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/06"

From OpenWetWare
Jump to: navigation, search
(Objective)
Line 20: Line 20:
 
*to run FPLC on the ADA protein from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/03| the third]] and [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/04 | the fourth]] of November.
 
*to run FPLC on the ADA protein from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/03| the third]] and [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/04 | the fourth]] of November.
  
 +
==Description==
 +
 +
*The cells from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/04 | the fourth]] of November were sonciated for 30 seconds and then placed in an ice bath for 30 seconds. This was repeated for a total of 3 times.
 +
*The cells were then transferred to centrifuge tubes, balanced, and placed in the centrifuge at 18,000rpm at 4°C for 2 hours.
 +
*The cells were then filtered using...
 +
*The cells were then run on FPLC, using the binding buffer and elusion buffer from [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/09/26| September 26th]].
 +
*The cells were collected in 5 mL aliquots and transferred into a Falcon tube.
 +
 +
 +
*The [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31|Au/Lysozyme solutions]] were obtained from the oven. The following images are the results from the Au/Lysozyme reaction.
 +
*[[Image:IMAG0851.jpg]]
 +
*[[Image:IMAG0852.jpg]]
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:15, 6 November 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/11/06</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Objective

  • to run transformations of PCR product into E. coli cells
  • to run FPLC on the ADA protein from the third and the fourth of November.

Description

  • The cells from the fourth of November were sonciated for 30 seconds and then placed in an ice bath for 30 seconds. This was repeated for a total of 3 times.
  • The cells were then transferred to centrifuge tubes, balanced, and placed in the centrifuge at 18,000rpm at 4°C for 2 hours.
  • The cells were then filtered using...
  • The cells were then run on FPLC, using the binding buffer and elusion buffer from September 26th.
  • The cells were collected in 5 mL aliquots and transferred into a Falcon tube.


  • The Au/Lysozyme solutions were obtained from the oven. The following images are the results from the Au/Lysozyme reaction.
  • IMAG0851.jpg
  • IMAG0852.jpg