Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/23"

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(Description)
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==Description==
 
==Description==
  
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''Lysozyme/Au Solutions''
 
*HAuCl<sub>4</sub> and Lysozyme solutions were prepared.  
 
*HAuCl<sub>4</sub> and Lysozyme solutions were prepared.  
 
**7.4mg  HAuCl<sub>4</sub> in 5.0 mL -->  
 
**7.4mg  HAuCl<sub>4</sub> in 5.0 mL -->  
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**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.
 
**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.
 
***The solutions bubbled over in the microwave. No fibers were formed. Next time, will try the same protocol as used when creating the Au/BSA mixtures in September.  
 
***The solutions bubbled over in the microwave. No fibers were formed. Next time, will try the same protocol as used when creating the Au/BSA mixtures in September.  
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 +
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''Preparing Solutions for Chemilluminescence''
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*0.564 mM luminol stock solution was prepared in carbonate buffer
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**0.0010 g luminol + 10 mL carbonate buffer --> 0.564 mM luminol solution.
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**Goal: 0.5mM solution
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*5 mM H<sub>2</sub>O<sub>2</sub> stock solution in carbonate buffer:
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**3.9 μL of 30 wt% H<sub>2</sub>O<sub>2</sub> + 10 mL of carbonate buffer --> 5 mM H<sub>2</sub>O<sub>2</sub>solution.
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*9.2 μM HRP solution
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**4.0 mg HRP + 1 mL '''autoclaved''' water --> 9.2 μM HRP solution.
  
 
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Revision as of 07:52, 25 October 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/23</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Objective

  • to create protein fibers in lysozyme/Au solutions
  • to prepare solutions for chemiluminescence reactions

Description

Lysozyme/Au Solutions

  • HAuCl4 and Lysozyme solutions were prepared.
    • 7.4mg HAuCl4 in 5.0 mL -->
    • 0.001g Lysozyme in 5.0 mL -->5mM
    • 8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles by Yeonju Lee and Kurt E. Geckeler.
      • The solutions bubbled over in the microwave. No fibers were formed. Next time, will try the same protocol as used when creating the Au/BSA mixtures in September.


Preparing Solutions for Chemilluminescence

  • 0.564 mM luminol stock solution was prepared in carbonate buffer
    • 0.0010 g luminol + 10 mL carbonate buffer --> 0.564 mM luminol solution.
    • Goal: 0.5mM solution
  • 5 mM H2O2 stock solution in carbonate buffer:
    • 3.9 μL of 30 wt% H2O2 + 10 mL of carbonate buffer --> 5 mM H2O2solution.
  • 9.2 μM HRP solution
    • 4.0 mg HRP + 1 mL autoclaved water --> 9.2 μM HRP solution.