Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/23"

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(Autocreate 2012/10/23 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
 
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==Objective==
 
==Objective==
  
#To make biocompatible protein films with specific reactive sites
+
*to create protein fibers in lysozyme/Au solutions
#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
+
*to prepare solutions for chemiluminescence reactions
  
 +
==Description==
 +
 +
*HAuCl<sub>4</sub> and Lysozyme solutions were prepared.
 +
**7.4mg  HAuCl<sub>4</sub> in 5.0 mL -->
 +
**0.001g Lysozyme in 5.0 mL -->5mM
 +
**8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper ''Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles'' by Yeonju Lee and Kurt E. Geckeler.
  
 
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Revision as of 06:14, 25 October 2012

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Objective

  • to create protein fibers in lysozyme/Au solutions
  • to prepare solutions for chemiluminescence reactions

Description

  • HAuCl4 and Lysozyme solutions were prepared.
    • 7.4mg HAuCl4 in 5.0 mL -->
    • 0.001g Lysozyme in 5.0 mL -->5mM
    • 8mL mixtures with mole ratios of 10, 15, 20, 25, 30, 35, and 40 Au/Lysozyme were made and microwaved for 2 minutes, based on a protocol from the paper Cytotoxicity and cellular uptake of lysozyme-stabilized gold nanoparticles by Yeonju Lee and Kurt E. Geckeler.