Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/17"

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(Autocreate 2012/10/17 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
 
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==Objective==
 
==Objective==
  
#To make biocompatible protein films with specific reactive sites
+
* to prepare and run RuATP column.
#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
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 +
==Description==
 +
*1.5L of 2:4:4:3 isopropanol:dioxane:water:25% ammonium hydride solution was made.
 +
*a [[User:Dhea_Patel/Notebook/Phosphorylation/2012/06/25| silica gel column]] was made to purify the RuATP. The isopropanol:dioxane:water:25% ammonium hydride was used as the solvent instead of acetonitrile. No variations in the solvent were used.
 +
 
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* purified Ru was dissolved in about 10mL of DMF before added to the ATP reaction.
  
  

Revision as of 09:02, 17 October 2012

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Objective

  • to prepare and run RuATP column.

Description

  • 1.5L of 2:4:4:3 isopropanol:dioxane:water:25% ammonium hydride solution was made.
  • a silica gel column was made to purify the RuATP. The isopropanol:dioxane:water:25% ammonium hydride was used as the solvent instead of acetonitrile. No variations in the solvent were used.
  • purified Ru was dissolved in about 10mL of DMF before added to the ATP reaction.