Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/09"

From OpenWetWare
Jump to: navigation, search
Line 20: Line 20:
  
 
==Description==
 
==Description==
 +
 +
[[User:Melissa_Novy/Notebook/CHEM-571/2012/10/09| Please refer to Melissa's entry for further details on running Au/BSA mixtures through the AAS]]
  
 
*Because the fibers were mixed and floating in the solution, some of the Au/BSA mixtures were filtered using 450nm pored-filter paper.  
 
*Because the fibers were mixed and floating in the solution, some of the Au/BSA mixtures were filtered using 450nm pored-filter paper.  

Revision as of 07:10, 25 October 2012

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/09</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Objective

  • To run atomic absorbance on Au/BSA samples

Description

Please refer to Melissa's entry for further details on running Au/BSA mixtures through the AAS

  • Because the fibers were mixed and floating in the solution, some of the Au/BSA mixtures were filtered using 450nm pored-filter paper.
    • The solutions that were filtered turned from a pinkish color (indicating the presence of gold) to a colorless solution.
  • All the mixtures were run through the AA.
  • The AA used 7 standards as controls.
  • The AA tubing was placed in the sample tube and allowed to burn the sample until a steady absorbance was obtained. Then, the tube was placed in de-ionized water until the next sample was ready to be run.
  • As predicted, the filtered mixtures showed little to no gold particles present in the solution, which means that the gold was filtered out along with the fibers.