Difference between revisions of "User:Dhea Patel/Notebook/Experimental Biological Chemistry Notebook/2012/10/03"

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(Autocreate 2012/10/03 Entry for User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook)
 
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==Objective==
 
==Objective==
  
#To make biocompatible protein films with specific reactive sites
+
*to purify filtered protein through FPLC and
#Explore the solution conditions (pH, ionic strength, buffer, stabilizers)  that produce stable protein – gold nanoparticle suspensions
+
*to remake Au/BSA solutions to run through Atomic Absorption.
  
 +
==Description==
  
 +
''' Purifying Protein via FPLC'''
 +
 +
 +
'''Preparing Au/BSA Solutions of AA'''
 +
*The stock solutions for HAuCl<sub>4</sub>  and BSA were combined using the following volumes
 
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__NOTOC__
 
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Revision as of 16:43, 3 October 2012

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Objective

  • to purify filtered protein through FPLC and
  • to remake Au/BSA solutions to run through Atomic Absorption.

Description

Purifying Protein via FPLC


Preparing Au/BSA Solutions of AA

  • The stock solutions for HAuCl4 and BSA were combined using the following volumes