Difference between revisions of "User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/04/03"

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(Entry title)
(Preparation of Reagents)
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==Preparation of Reagents==
 
 
* 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4.
 
* A new stock solution of 200 μM adenosine stock solution was also prepared.
 
* For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM.
 
* Adenosine trials were performed at 265 nm. A histogram of that trial is shown below.
 
 
[[Image:untitledx.png|center]]
 
 
  
 
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Revision as of 13:14, 8 May 2013

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