User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13

From OpenWetWare
Jump to: navigation, search

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Objective

  • to finish Trail 2 and run Trial 3 of ADA kinetics

Description

  • to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.
    • keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.
  • The following table was used to create the mixtures in the cuvette.
    • Data Table.JPG
    • In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
    • All the listed concentrations were run for trial 3.

Data

Average Lineweaver-Burk Plot.png

Average Velocities.png

Screen Shot 2013-02-19 at 11.14.56 AM.png