User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13

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  • to finish Trail 2 and run Trial 3 of ADA kinetics


  • to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.
    • keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.
  • The following table was used to create the mixtures in the cuvette.
    • Data Table.JPG
    • In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
    • All the listed concentrations were run for trial 3.