Difference between revisions of "User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13"

From OpenWetWare
Jump to: navigation, search
m (Description)
m
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
{|{{table}} width="800"
 
{|{{table}} width="800"
 
|-
 
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #cdde95;" align="center"|
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
<!-- ## START calendar column -->
|-
+
<LNCalendar></LNCalendar>
| colspan="2"|
+
<!-- ## END calendar column -->
 +
|align="center" style="background-color: #e5edc8;" |
 +
<!-- ## START search column: Place your logo here. Try keep it below 200px in width and 150px in height. ##  -->
 +
[[Image:BDLlogo_300.png|300px]] <sitesearch>title=Search this Project</sitesearch>
 +
<!-- ## END search column  ## -->
 +
|-
 +
|colspan="2" style="background-color: #F2F2F2;" align="right"|[[{{FULLPAGENAME}}/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
 +
|-  
 +
|colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
=+Objective==
+
 
 +
==Objective==
 
*to finish Trail 2 and run Trial 3 of ADA kinetics
 
*to finish Trail 2 and run Trial 3 of ADA kinetics
  
Line 16: Line 25:
 
**In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
 
**In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
 
**All the listed concentrations were run for trial 3.
 
**All the listed concentrations were run for trial 3.
 +
 +
==Data==
 +
[[Image:Average_Lineweaver-Burk_Plot.png]]
 +
 +
[[Image:Average_Velocities.png]]
 +
 +
[[Image:Screen_Shot_2013-02-19_at_11.14.56_AM.png]]
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 09:16, 19 February 2013

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext"></div><div style="display:none;" id="page">User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/13</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>

Objective

  • to finish Trail 2 and run Trial 3 of ADA kinetics

Description

  • to baseline UV-vis, have one cuvette of phosphate buffer in reference cell and one cuvette of phosphate buffer in sample cell and run baseline from 200-400nm.
    • keep the cuvette containing phosphate buffer in the reference cell and put the adenosine + buffer + ADA in the sample cell.
  • The following table was used to create the mixtures in the cuvette.
    • Data Table.JPG
    • In order to complete trail 2, 100uM, 150uM and 200uM [adenosine] were run.
    • All the listed concentrations were run for trial 3.

Data

Average Lineweaver-Burk Plot.png

Average Velocities.png

Screen Shot 2013-02-19 at 11.14.56 AM.png