Difference between revisions of "User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05"
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*Once it was determined that 100μM adenosine created a clear spectra, kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table above). | *Once it was determined that 100μM adenosine created a clear spectra, kinetics was run at 265nm using 2.38mL phosphate buffer, 600μL of 500μM stock adenosine, and 20μL ADA (as outlined in the table above). | ||
**The velocity was determined by clicking Operations > Activity Table > dAbs. The average of the dAbs values is the average slope (velocity). | **The velocity was determined by clicking Operations > Activity Table > dAbs. The average of the dAbs values is the average slope (velocity). | ||
+ | *Then, an assay was run at 265nm using 2.38mL phosphate buffer, 600μL of 50μM stock adenosine, and 20μL ADA (as outlined in the table above). | ||
+ | **The absorbance was close to 0, indicating that the ideal adenosine concentration ranges between 1μM and 10μM. | ||
==Observations== | ==Observations== |
Revision as of 12:28, 5 February 2013
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Objective
Calculations
Procedure
Observations |