Difference between revisions of "User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23"

From OpenWetWare
Jump to: navigation, search
(Objective)
Line 14: Line 14:
 
|colspan="2"|
 
|colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Objective==
 
 
*To complete project proposal
 
*To complete experimental protocol for next week.
 
  
  

Revision as of 12:48, 8 May 2013

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">02/05/2013</div><div style="display:none;" id="page">User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

BDLlogo 300.png <sitesearch>title=Search this Project</sitesearch>

Customize your entry pages <html><img src="/images/a/aa/Help.png" border="0" /></html>


Experimental Protocol

Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)

  • Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L.
    • The pH of the final solution will be 7.4.
    • This buffer can be stored for up to 1 mo at 4°C.
  • Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

Running ADA Kinetics

  • The following table outlines the concentrations and volumes of the solutions used in the ADA kinetics assay to be performed next Tuesday.

px80

  • To prepare a 30 mM stock solution of adenosine:

Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{0.030mol}{L}} of adenosine × Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{267.24 g}{1 mol}} = Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{8.0172g}{L}} of adenosine in buffer

  • To make 10mL of solution, 0.080172g of adenosine will be added to 10mL of phosphate buffer.