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| |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> |
| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} |
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| ==Aspirin concentration for ADA Kinetic Assay==
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| * The assay for the concentrations below will be conducted next laboratory period.
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| [[Image:Screen_Shot_2013-02-19_at_1.13.00_PM.png|center]]
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| ==ADA Kinetic Assay for obtaining the zero point==
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| * The procedure was taken from [[User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/20|Mary Mendoza.]]
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| * The objective of this laboratory period is to conduct adenosine deaminase (ADA) kinetic assay runs for the new calculated concentrations.
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| * UV 2550 Shimadzu spectrophotometer was baseline with 0.05 M sodium phosphate buffer.
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| * The assays were prepared according to the data below.
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| [[Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png|center]]
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| * After running the first trial, it was observed that the absorbance for 12.34 μM adenosine of trial 2 was superimposed over the 10.52 μM adenosine of trial 1.
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| * It was suggested by Dr. Hartings to use Beer's Law for accurate measurement of the concentration of adenosine in solution.
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| * An article was provided by Dr. Hartings with the molar extinction coefficient, 1.53 x 10<sup>-4</sup> of adenosine at 260 nm.
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| * Thus, it was decided to run a full spectrum of adenosine at each given concentration specified above for accurate measurement of the concentration of adenosine.
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| ==Data==
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| [[Image:Concen1.png|center]]
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| [[Image:AveVelo1.png|center]]
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| [[Image:1adeno.png|center]]
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| [[Image:Lin1.png|center]]
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| [[Image:UV2.20.png|center]]
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Project name
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