User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/12: Difference between revisions
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*Temperature control: 25°C | *Temperature control: 25°C | ||
*Phosphate buffer in reference cell | *Phosphate buffer in reference cell | ||
*Ran kinetics on 250uM solution (20uL ADA, 600uL 1250uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm. | |||
**absorbance exceeded 4.00, so the assay was stopped and a new solution was prepared. | |||
*Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12.8uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm. | *Ran kinetics on 2.56uM solution (20uL ADA, 600uL 12.8uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm. | ||
* | **dAbs was obtained and plotted vs. time in excel | ||
*Ran kinetics on 6.4uM solution (20uL ADA, 600uL 32uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm. | |||
**dAbs was obtained and plotted vs. time in excel | |||
*Ran kinetics on 16uM solution (20uL ADA, 600uL 80uM Adenosine, and 2.38mL pH7.4, 0.005M phosphate buffer) for 5 minutes at 265nm. | |||
**dAbs was obtained and plotted vs. time in excel | |||
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Revision as of 11:15, 12 February 2013
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