User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/05: Difference between revisions
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== | ==Objective== | ||
* | *to perform ADA kinetics assays using UV-vis. | ||
==Calculations== | |||
*ADA Kinetics were run using the following volumes and concentrations. | |||
**Note that the table has been adjusted based on the change in concentration of the adenosine stock solution. | |||
[[Image:Screen_Shot_2013-02-05_at_11.59.42_AM.png]] | |||
*The kinetics was run using UV-vis at 25°C at 265nm for 5 minutes. | |||
==Procedure== | |||
#UVProbe was opened. | |||
##Window > 1. Kinetics | |||
##Methods Icon | |||
###Wavelength: 265nm | |||
###Duration: 600 seconds (5minutes) | |||
###OK | |||
#Shimadzu CPS-Controller was set to 25°C. | |||
##wait for the temperature to raise to 25°C | |||
#place the sample in the cell and click start. | |||
==Observations== | |||
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Revision as of 10:46, 5 February 2013
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Objective
Calculations
Procedure
Observations |