Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/15"

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(Autocreate 2013/08/15 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
 
(Remains of the Day)
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==Remains of the Day==
 
==Remains of the Day==
* Write up your awesome plans and results here...
+
* Crap, the Oomycete PCR seem to be getting worse. After dramatically upping the MgCl2 (4 ul of 50 mM MgCl2 rather than 2) the reactions with OomF and OomR failed worse than ever, with lots of weird smears. Gonna try the nested approach again.  
  
  

Revision as of 14:15, 16 August 2013

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Remains of the Day

  • Crap, the Oomycete PCR seem to be getting worse. After dramatically upping the MgCl2 (4 ul of 50 mM MgCl2 rather than 2) the reactions with OomF and OomR failed worse than ever, with lots of weird smears. Gonna try the nested approach again.