Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/09"

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(Remains of the Day)
(Remains of the Day)
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* Repeat tomato plant spray with Actigard and TBG products
 
* Repeat tomato plant spray with Actigard and TBG products
 
* Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday
 
* Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday
* Run gels of nested Oomycete PCRs
+
* Run gels of nested Oomycete PCRs (looks like a good approach, but using experience from 2013/06/27, I should do 40 cycles, have more MgCl2 and use 57 degree annealing rather than 62)
  
 
[[Image:August9,2013Gel.tif]]
 
[[Image:August9,2013Gel.tif]]

Revision as of 11:10, 9 August 2013

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Remains of the Day

  • Repeat tomato plant spray with Actigard and TBG products
  • Streak out plates of P. syringae DC95-5 to inoculate tomato leaves with on Monday
  • Run gels of nested Oomycete PCRs (looks like a good approach, but using experience from 2013/06/27, I should do 40 cycles, have more MgCl2 and use 57 degree annealing rather than 62)

File:August9,2013Gel.tif