Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/08/08"

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(Remains of the Day)
(Remains of the Day)
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* Get figures together for Australia report
 
* Get figures together for Australia report
 
* Prepare Earth Alive figures
 
* Prepare Earth Alive figures
 +
* Since the Oomycete gradient PCR didn't appear to say the problem was temperature (see below), I'm gonna try a nested approach to get amplifiable sequence for oomycete TRFLP in samples where there isn't much. So I used the DC6 primer in combo with OomR plus 5x25 ul of MgCl per 50 ul reaction. 35 cycles and 62 degree annealing. Will take 1 ul of this and apply it to OomF and OomR reaction, run under same conditions for a total of 70 cycles of amplification. Let see on gels tommorow how well this approach works.
  
 
[[Image:August8,2013Gel.tif]]
 
[[Image:August8,2013Gel.tif]]

Revision as of 10:33, 8 August 2013

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Remains of the Day

  • Gonna start writing introduction to TVD paper (copy bits from previous TVD reports)
  • Get figures together for Australia report
  • Prepare Earth Alive figures
  • Since the Oomycete gradient PCR didn't appear to say the problem was temperature (see below), I'm gonna try a nested approach to get amplifiable sequence for oomycete TRFLP in samples where there isn't much. So I used the DC6 primer in combo with OomR plus 5x25 ul of MgCl per 50 ul reaction. 35 cycles and 62 degree annealing. Will take 1 ul of this and apply it to OomF and OomR reaction, run under same conditions for a total of 70 cycles of amplification. Let see on gels tommorow how well this approach works.

File:August8,2013Gel.tif