Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/07/30"

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(Remains of the Day)
(Remains of the Day)
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==Remains of the Day==
 
==Remains of the Day==
 
* Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
 
* Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
* Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time.  
+
* Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time. He did seem to be interested in the ommycete tests I did however so I sent him stuff on that.  
  
  

Revision as of 15:53, 30 July 2013

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Remains of the Day

  • Ran TRFLP of Oomycetes on previous PCR of the California strawberry samples, but it didn't work, amplifying primer dimers exclusively. May repeat on a subset using a much higher annealing temperaute (ie 65 vs 56) or with the straight up DNA but using more cycles (ie. 55 vs 44).
  • Got email back from Joji. Turns out they didn't need or intend to use the soil chemistry information I sent over. Wikid, I love wasting my time. He did seem to be interested in the ommycete tests I did however so I sent him stuff on that.