Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/06/20"

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(Autocreate 2013/06/20 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Remains of the Day==
 
==Remains of the Day==
* Write up your awesome plans and results here...
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* Gonna combine optimized Oomycete DC6 primer tagged with 6FAM (OomF DavDC6 – GAGGGACTTTtGGGTAATCA) with OomR for about 1600 bp TRFLP amplicons and then use the below primers for nested detection of particular species.  
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(Actually ordered Pythium irregulare: PythIrreg Fwd – CGGAGCGGCGGGTGCTGTK with PythIrreg Rev – CaCCGAagtcgCcgACAG) [adapted from, “Species-specific PCR primers for Pythium developed from ribosomal ITS1 region”]
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(Actually ordered Pythium ultimum: PythUltim Fwd – ACGAAGGTTGGTCTGTTG with PythUltim Rev – TCTCTACGCAACTAAATGC) [adapted from, “DETECTION OF PYTHIUM ULTIMUM FROM SOIL USING PCR MICROPLATE HYBRIDIZATION WITH SPECIES-SPECIFIC PRIMERS”]
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(Actually ordered Phytophthora fragariae: PhytopFrag Fwd– with cACTTAGTTGGGGGCCTGTC with PhytopFrag Rev – CGCCGACTGGCCACACAG) [adapted from, “Validation and Exploitation of a PCR-based Diagnostic for Detecting Phytophthora in Strawberry and Raspberry”]
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(Actually ordered Phytophthora infestans: PhytopInfest Fwd – TGGGCGAGCCCTATCAAAA  with PhytopInfest Rev – CGATTCAAATGCCAAGCTA)[adapted from, “Development of a species-specific and sensitive detectionassay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil”]
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Also gonna order fresh 27F Degen with a 6FAM label on it: AGRRTTYGATYHTGGYTYAG (noted that I put a typo into the sequence I used in my PhD)
  
  

Latest revision as of 21:46, 26 September 2017

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Remains of the Day

  • Gonna combine optimized Oomycete DC6 primer tagged with 6FAM (OomF DavDC6 – GAGGGACTTTtGGGTAATCA) with OomR for about 1600 bp TRFLP amplicons and then use the below primers for nested detection of particular species.

(Actually ordered Pythium irregulare: PythIrreg Fwd – CGGAGCGGCGGGTGCTGTK with PythIrreg Rev – CaCCGAagtcgCcgACAG) [adapted from, “Species-specific PCR primers for Pythium developed from ribosomal ITS1 region”]

(Actually ordered Pythium ultimum: PythUltim Fwd – ACGAAGGTTGGTCTGTTG with PythUltim Rev – TCTCTACGCAACTAAATGC) [adapted from, “DETECTION OF PYTHIUM ULTIMUM FROM SOIL USING PCR MICROPLATE HYBRIDIZATION WITH SPECIES-SPECIFIC PRIMERS”]

(Actually ordered Phytophthora fragariae: PhytopFrag Fwd– with cACTTAGTTGGGGGCCTGTC with PhytopFrag Rev – CGCCGACTGGCCACACAG) [adapted from, “Validation and Exploitation of a PCR-based Diagnostic for Detecting Phytophthora in Strawberry and Raspberry”]

(Actually ordered Phytophthora infestans: PhytopInfest Fwd – TGGGCGAGCCCTATCAAAA with PhytopInfest Rev – CGATTCAAATGCCAAGCTA)[adapted from, “Development of a species-specific and sensitive detectionassay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil”]

Also gonna order fresh 27F Degen with a 6FAM label on it: AGRRTTYGATYHTGGYTYAG (noted that I put a typo into the sequence I used in my PhD)