Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/05/03"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
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|style="background-color: #EEM"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Remains of the Day==
 
==Remains of the Day==
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* Stephanie finished extraction of rhizosphere DNA from trial 2 of the strawberry experiment (Albion speedlings grown in growth room with diffferent washes and bacterial innoculations)
 
* Growing 2 L of the Pseudomonas for tomatoes for George (at room temp with gentle shaking over the weekend)
 
* Growing 2 L of the Pseudomonas for tomatoes for George (at room temp with gentle shaking over the weekend)
 
* Mixed 1 ml of the below overnights in R2A with 1 mL of water with 0.2% silwett and am inoculating 20 ul onto tomato leaves since no other symptoms appear to be coming (put these tubes into the 4 degree and innoculated on Sunday - 20 ul per leaf with a mix up and inoculation of Pst T6D1 onto the Pst DC01 treatment and no inoculation of the Pst T6D1 treatment (so this treatment was only inoculated the one time like two weeks ago))
 
* Mixed 1 ml of the below overnights in R2A with 1 mL of water with 0.2% silwett and am inoculating 20 ul onto tomato leaves since no other symptoms appear to be coming (put these tubes into the 4 degree and innoculated on Sunday - 20 ul per leaf with a mix up and inoculation of Pst T6D1 onto the Pst DC01 treatment and no inoculation of the Pst T6D1 treatment (so this treatment was only inoculated the one time like two weeks ago))
 
  
 
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! width="50"|Disease caused || width="50"|Species/Strain || width="50"|OD600  
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| width="50"|Disease caused || width="50"|Species/Strain || width="50"|OD600  
 
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| Bacterial speck || P. syringae DC01 || 1.32||  
 
| Bacterial speck || P. syringae DC01 || 1.32||  

Revision as of 08:07, 17 July 2013

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Owwnotebook icon.png Applied Soil Microbial Ecology <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Remains of the Day

  • Stephanie finished extraction of rhizosphere DNA from trial 2 of the strawberry experiment (Albion speedlings grown in growth room with diffferent washes and bacterial innoculations)
  • Growing 2 L of the Pseudomonas for tomatoes for George (at room temp with gentle shaking over the weekend)
  • Mixed 1 ml of the below overnights in R2A with 1 mL of water with 0.2% silwett and am inoculating 20 ul onto tomato leaves since no other symptoms appear to be coming (put these tubes into the 4 degree and innoculated on Sunday - 20 ul per leaf with a mix up and inoculation of Pst T6D1 onto the Pst DC01 treatment and no inoculation of the Pst T6D1 treatment (so this treatment was only inoculated the one time like two weeks ago))
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Disease caused Species/Strain OD600
Bacterial speck P. syringae DC01 1.32
Bacterial speck P. syringae DC08 1.18
Bacterial speck P. syringae DC95 0.86
Bacterial speck P. syringae T6D1 1.25
Bacterial spot X. gardneri 444 1.07
Bacterial spot X. gardneri DC00 NA
Bacterial spot X. gardneri DC03 NA
Bacterial spot X. gardneri 1B5B 1.51