User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/12

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Remains of the Day

  • Paint bacterial speck and spot suspensions onto plants and incubate for two weeks. - did not do, perhaps Tuesday when other true leaves start to emerge
  • Sequences failed cause the M13 Forward primer falls outside the amplified region. Must use T7 or SP6 to sequence.
  • Made virtual TRFLP for individual strains used in the strawberry innoculation experiment. Only enterobacter are really detectable with those primers. :-/