Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/03"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
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==Remains of the Day==
 
==Remains of the Day==
* Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 primers.  
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* Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 Forward primer - sent as 1 - 32 in the order they were run on the below gel (remember it was multichannel so 1-8 from the first strip and staggered with 9-16 and etc).
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* Cut potatoes for innoculation with the rest of the endophytes tommorow.
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* Found good article about induction of SAR with innoculation with Methylobacteria (http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046802#pone.0046802-Rosenblueth1)
  
 
[[image:April 3, 2013Gel.tif]]
 
[[image:April 3, 2013Gel.tif]]

Latest revision as of 21:35, 26 September 2017

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Remains of the Day

  • Ran gel of yesterday's PCR and most colony PCRs worked. Will purify and send for sequencing using M13 Forward primer - sent as 1 - 32 in the order they were run on the below gel (remember it was multichannel so 1-8 from the first strip and staggered with 9-16 and etc).
  • Cut potatoes for innoculation with the rest of the endophytes tommorow.

File:April 3, 2013Gel.tif