Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/03/25"

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(Remains of the Day)
(Remains of the Day)
 
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* Day is 32 days since TBG potato tissue trial started. Harvest, photograh, and weigh plantlets - done.
 
* Day is 32 days since TBG potato tissue trial started. Harvest, photograh, and weigh plantlets - done.
  
* Ran gel of NRC TVD Rhizospheres. Looks like there's useable signal for all, so gonna digest with HhaI (bacteria) and DdeI (fungus) without PCR purification. Here's a pic of the gel:
+
* Ran gel of NRC TVD Rhizospheres. Looks like there's useable signal for all, so gonna digest with HhaI (bacteria) and DdeI (fungus) overnight at 35 degrees without PCR purification. Here's a pic of the gel:
  
 
[[image:March25,2013Gel.tif]]
 
[[image:March25,2013Gel.tif]]

Latest revision as of 16:00, 25 March 2013

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Remains of the Day

  • Day is 32 days since TBG potato tissue trial started. Harvest, photograh, and weigh plantlets - done.
  • Ran gel of NRC TVD Rhizospheres. Looks like there's useable signal for all, so gonna digest with HhaI (bacteria) and DdeI (fungus) overnight at 35 degrees without PCR purification. Here's a pic of the gel:

File:March25,2013Gel.tif