Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/03/23"

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(Remains of the Day)
(Remains of the Day)
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==Remains of the Day==
 
==Remains of the Day==
 
* Ran TRLP PCRs for bacteria and fungus on the 1, 7, 8, 9B and 9G samples of the tomatoe rhizospheres sent from NRC Sask
 
* Ran TRLP PCRs for bacteria and fungus on the 1, 7, 8, 9B and 9G samples of the tomatoe rhizospheres sent from NRC Sask
* Ran gel of physical capture TRFLP. Poor separation of large frags perhaps cause BstNI doesn't cut a lot. Looks great otherwise:  
+
* Ran gel of physical capture TRFLP. Poor separation of large frags perhaps cause BstNI doesn't cut a lot. Cut out everything that resolved as a single band and will sequence some on Monday. Looks great otherwise:  
  
 
[[image:March23,2013Gel.tif]]
 
[[image:March23,2013Gel.tif]]

Revision as of 20:41, 23 March 2013

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Remains of the Day

  • Ran TRLP PCRs for bacteria and fungus on the 1, 7, 8, 9B and 9G samples of the tomatoe rhizospheres sent from NRC Sask
  • Ran gel of physical capture TRFLP. Poor separation of large frags perhaps cause BstNI doesn't cut a lot. Cut out everything that resolved as a single band and will sequence some on Monday. Looks great otherwise:

File:March23,2013Gel.tif