Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/02/19"

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(Remains of the Day)
(Remains of the Day)
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==Remains of the Day==
 
==Remains of the Day==
 
* Ran gel of yesterday's fungal PCR (those with stars beside the name will be repeated with 40 cycles/TVD fields will all be repeated with 40):  
 
* Ran gel of yesterday's fungal PCR (those with stars beside the name will be repeated with 40 cycles/TVD fields will all be repeated with 40):  
* None of the replacement PCRs that I did for day before yesterday's bacterial TRFLP worked. This time, repeating with 50 ul reaction volume and 5 extra cycles.  
+
* None of the replacement PCRs that I did for day before yesterday's bacterial TRFLP worked. This time, repeating with 50 ul reaction volume and 5 extra cycles (as well as for all failed fungal reactions and all TVD field fungal).  
 
* Got Steph started on culturing microbes for the strawberry rhizosphere experiment.  
 
* Got Steph started on culturing microbes for the strawberry rhizosphere experiment.  
 +
* Extracted 23 more TVD growth room rep 2 rhizospheres
  
 
[[image:Feb19,2013Gel.tif]]
 
[[image:Feb19,2013Gel.tif]]

Revision as of 18:38, 19 February 2013

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Remains of the Day

  • Ran gel of yesterday's fungal PCR (those with stars beside the name will be repeated with 40 cycles/TVD fields will all be repeated with 40):
  • None of the replacement PCRs that I did for day before yesterday's bacterial TRFLP worked. This time, repeating with 50 ul reaction volume and 5 extra cycles (as well as for all failed fungal reactions and all TVD field fungal).
  • Got Steph started on culturing microbes for the strawberry rhizosphere experiment.
  • Extracted 23 more TVD growth room rep 2 rhizospheres

File:Feb19,2013Gel.tif