Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/02/05"

From OpenWetWare
Jump to: navigation, search
(Remains of the Day)
(Remains of the Day)
Line 11: Line 11:
 
*Did Oomycete TRFLP primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and infected arrugula rhizosphere as a positive control,  and Alliston potato soil DNA as a test.  
 
*Did Oomycete TRFLP primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and infected arrugula rhizosphere as a positive control,  and Alliston potato soil DNA as a test.  
 
*Autoclaved LGI broth for use in plate incubation test tommorow.
 
*Autoclaved LGI broth for use in plate incubation test tommorow.
 +
* Did PCR for fungal trflp of California soils that Joseph extracted since he's been having a hard time doing it.
  
  

Revision as of 15:56, 5 February 2013

Owwnotebook icon.png Applied Soil Microbial Ecology <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Remains of the Day

  • Interviewed intern candidates most of the day
  • Did NifH primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and Klebsiella pneumonii as a positive control, E. coli as negative, Methylobacterial cells as test and corn soil DNA as a test.
  • Did Oomycete TRFLP primer test, as gradient from 52 to 62 degrees, with 40 cycles, 2.5 minutes extension, 25 ul of reaction and infected arrugula rhizosphere as a positive control, and Alliston potato soil DNA as a test.
  • Autoclaved LGI broth for use in plate incubation test tommorow.
  • Did PCR for fungal trflp of California soils that Joseph extracted since he's been having a hard time doing it.