User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/12/06: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
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==Remains of the Day==
==Remains of the Day==
* Write up your awesome plans and results here...
* Ran gel of yesterday's bacterial PCR for TRFLP testing. At question is whether non-purified samples are as good as purified samples for TRFLP analysis when the reaction is saturated by additional rounds of amplification. They were'nt when it was only 30 cycles, but maybe now they'll contain as many peaks cause of PCR saturation.  
 
[[image: December 6,2012Gel.tif]]




Latest revision as of 22:19, 26 September 2017

Applied Soil Microbial Ecology Main project page
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Remains of the Day

  • Ran gel of yesterday's bacterial PCR for TRFLP testing. At question is whether non-purified samples are as good as purified samples for TRFLP analysis when the reaction is saturated by additional rounds of amplification. They were'nt when it was only 30 cycles, but maybe now they'll contain as many peaks cause of PCR saturation.



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