Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/11/29"

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(Autocreate 2012/11/29 Entry for User:David_Johnston_Monje/Notebook/Maize_Endophyte_Biofertilizers)
 
(Remains of the Day)
 
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==Remains of the Day==
 
==Remains of the Day==
* Write up your awesome plans and results here...
+
* Plated HAW 1658, HHA 4037, OKA plants today (extracted them yesterday). This time, I plated 10X dilution for both roots and shoots. Also, I washed with water after the final ethanol wash, so it might help keep the cells alive cause I think I over sterlized the guys on Tuesday. Maybe will have to repeat the whole experiment with a single plant per genotype and much lighter sterilization (like just a single wash of bleach with surfactant).
  
  

Latest revision as of 15:31, 29 November 2012

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Remains of the Day

  • Plated HAW 1658, HHA 4037, OKA plants today (extracted them yesterday). This time, I plated 10X dilution for both roots and shoots. Also, I washed with water after the final ethanol wash, so it might help keep the cells alive cause I think I over sterlized the guys on Tuesday. Maybe will have to repeat the whole experiment with a single plant per genotype and much lighter sterilization (like just a single wash of bleach with surfactant).