User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/11/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
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==Remains of the Day==
==Remains of the Day==
* Write up your awesome plans and results here...
* Plated HAW 1658, HHA 4037, OKA plants today (extracted them yesterday). This time, I plated 10X dilution for both roots and shoots. Also, I washed with water after the final ethanol wash, so it might help keep the cells alive cause I think I over sterlized the guys on Tuesday. Maybe will have to repeat the whole experiment with a single plant per genotype and much lighter sterilization (like just a single wash of bleach with surfactant).




Latest revision as of 22:18, 26 September 2017

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Remains of the Day

  • Plated HAW 1658, HHA 4037, OKA plants today (extracted them yesterday). This time, I plated 10X dilution for both roots and shoots. Also, I washed with water after the final ethanol wash, so it might help keep the cells alive cause I think I over sterlized the guys on Tuesday. Maybe will have to repeat the whole experiment with a single plant per genotype and much lighter sterilization (like just a single wash of bleach with surfactant).



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