Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2012/11/27"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Applied Soil Microbial Ecology </span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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* Making glycerol stocks of rhizospheres, roots and shoots for potato endophyte experiment - shoots are unlickley to be interesting but do them anyway.  
 
* Making glycerol stocks of rhizospheres, roots and shoots for potato endophyte experiment - shoots are unlickley to be interesting but do them anyway.  
 
* After culturing and making glycerol stocks, freeze the falcon tubes for later DNA extraction
 
* After culturing and making glycerol stocks, freeze the falcon tubes for later DNA extraction
* Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of 10X andd 100X diluted mash each third of a genotype plate) and shoots (just the 10X dilution) on plates divided into 3 and grouped by genotype. Also innoculating the 10X diluted stuff into semi-solid LGI.
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* Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of 100X diluted mash each third of a genotype plate) and shoots (just the 10X dilution) on plates divided into 3 and grouped by genotype. Also innoculating the into semi-solid LGI (50 ul of 10X diluted stuff for shoot, 50 ul of 100X diluted stuff for roots).
  
 
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Latest revision as of 21:17, 26 September 2017

Owwnotebook icon.png Applied Soil Microbial Ecology Report.pngMain project page
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Remains of the Day

  • Making glycerol stocks of rhizospheres, roots and shoots for potato endophyte experiment - shoots are unlickley to be interesting but do them anyway.
  • After culturing and making glycerol stocks, freeze the falcon tubes for later DNA extraction
  • Weighed mash collected, and added twice as much phosphate buffer to each (ie. 1 g of mash recieved 2 mL). Plating roots (50 ul of 100X diluted mash each third of a genotype plate) and shoots (just the 10X dilution) on plates divided into 3 and grouped by genotype. Also innoculating the into semi-solid LGI (50 ul of 10X diluted stuff for shoot, 50 ul of 100X diluted stuff for roots).