User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/03/07

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03/07/13

HEK293 Luc #4 fixed DAPI staining


  • DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH2O
  • Diluted Hoescht to working concentration (1x) in 1xPBS.
  • Fixing cells with formaldehyde
  • Adding Hoescht to the cells, let sit in for 10 mins in the dark to develop signal

Results:

  • Good DAPI signal
  • Detection Algorithm failed due to very high cell density

Next steps:

  • Growing and imaging cells in less dense conditions