Difference between revisions of "User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/03/07"

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(Autocreate 2013/03/07 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern)
 
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==Entry title==
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==03/07/13==
* Insert content here...
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HEK293 Luc #4 fixed DAPI staining
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----
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* DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH<sub>2</sub>O
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* Diluted Hoescht to working concentration (1x) in 1xPBS.  
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* Fixing cells with formaldehyde
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* Adding Hoescht to the cells, let sit in for 10 mins in the dark to develop signal
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----
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Results:
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* Good DAPI signal
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* Detection Algorithm failed due to very high cell density
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----
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Next steps:
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* Growing and imaging cells in less dense conditions
  
  

Revision as of 21:01, 18 March 2013

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03/07/13

HEK293 Luc #4 fixed DAPI staining


  • DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH2O
  • Diluted Hoescht to working concentration (1x) in 1xPBS.
  • Fixing cells with formaldehyde
  • Adding Hoescht to the cells, let sit in for 10 mins in the dark to develop signal

Results:

  • Good DAPI signal
  • Detection Algorithm failed due to very high cell density

Next steps:

  • Growing and imaging cells in less dense conditions