User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/26

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02/26/13

HEK293 GAL-4 EED DAPI staining



  • DAPI/Hoescht: made by Dr. Haynes; 5000x in sterile dH2O
  • Diluted Hoescht to working concentration (1x) in 1xPBS.
  • Added 2 mL to each well
  • Let sit at room temperature for 5 mins in the dark



Results:

  • strong fluorescence signal only in dead/detached cells
  • very weak signal in living cells

Next steps:

  • Testing if longer dwell time prior to imaging improves DAPI signal
  • assay the possibility of different dye for nucleus staining in replicating cells
  • assay the possibilities to detect luciferase signal