User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/15

From OpenWetWare
< User:David Dreher‎ | Notebook‎ | Chromatin controlled cell pattern‎ | 2013‎ | 02
Revision as of 17:30, 18 February 2013 by Karmella Haynes (talk | contribs) (Entry title)
Jump to: navigation, search
Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

02/15/13

  • HEK293-luc cell culturing, trial 2



HEK293-luc #4 cell culturing, trial 2

  • Tried plating at the optimal density (1600 cells/well) in a 6-well plate; cells did not grow very well
  • Retry 6-well plates and also try 96-well plate
  • Made three new plates of HEK293-luc #4 cells
  • Started with ~1x106 harvested cells (confluent T25 flask)
  • Made the following dilutions:
    • (1) 1:25 dilution of harvested cells = 40,000 cells/mL 10% FBS DMEM
    • (2) 1:25 dilution of tube 1 = 1:625 dilution = 1,600 cells/mL 10% FBS DMEM
    • (3) 1:100 dilution of 1x106 cells/mL
  1. 6-well plate: 2 mL , 80,000 cells/ well
  2. 6-well plate: 2 mL 10% FBS DMEM, 3200 cells/ well