Difference between revisions of "User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/15"

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==Entry title==
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==02/15/13==
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* HEK293-luc cell culturing, trial 2
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''HEK293-luc #4 cell culturing, trial 2''
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* Tried plating at the optimal density (1600 cells/well) in a 6-well plate; cells did not grow very well
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* Retry 6-well plates and also try 96-well plate
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* Made three new plates of HEK293-luc #4 cells
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* Started with ~1x10<sup>6</sup> harvested cells (confluent T25 flask)
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* Made the following dilutions:
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**(1) 1:25 dilution of harvested cells = 40,000 cells/mL 10% FBS DMEM
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**(2) 1:25 dilution of tube 1 = 1:625 dilution = 1,600 cells/mL 10% FBS DMEM
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**(3) 1:100 dilution of 1x10<sup>6 cells/mL
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# 6-well plate: 2 mL , 80,000 cells/ well
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# 6-well plate: 2 mL 10% FBS DMEM, 3200 cells/ well
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Revision as of 17:30, 18 February 2013

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02/15/13

  • HEK293-luc cell culturing, trial 2



HEK293-luc #4 cell culturing, trial 2

  • Tried plating at the optimal density (1600 cells/well) in a 6-well plate; cells did not grow very well
  • Retry 6-well plates and also try 96-well plate
  • Made three new plates of HEK293-luc #4 cells
  • Started with ~1x106 harvested cells (confluent T25 flask)
  • Made the following dilutions:
    • (1) 1:25 dilution of harvested cells = 40,000 cells/mL 10% FBS DMEM
    • (2) 1:25 dilution of tube 1 = 1:625 dilution = 1,600 cells/mL 10% FBS DMEM
    • (3) 1:100 dilution of 1x106 cells/mL
  1. 6-well plate: 2 mL , 80,000 cells/ well
  2. 6-well plate: 2 mL 10% FBS DMEM, 3200 cells/ well