User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/06: Difference between revisions
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* | * HEK293-luc14 plate: 6-well plate | ||
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'''HEK293-luc14 plate''' | |||
* Plated HEK293-luc14 positive control cells at optimal density (1.6x10<sup>3</sup>), as determined by previous dilution plating | |||
** Tryspinized cells, brought conc. to 1x10<sup>6</sup>/mL | |||
** Added 0.5 mL to 12 mL medium (1/25 dilution) | |||
** Added 0.5 mL of 1/25 dilution to 12 mL medium (1/625 dilution) | |||
** Added 1 mL of the 1/625 dilution to each well | |||
** Added an additional 1 mL growth medium to each well (to prevent the medium from drying out) | |||
* Grow for 7 days to get colonies | |||
* Will stain with Hoescht (DAPI) for fluorescent imaging; maybe try D-luciferin when it arrives. | |||
Revision as of 19:34, 6 February 2013
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02/06/13
HEK293-luc14 plate
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