User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/02/04

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Fourth observation on cell growth for dilution sequence

Well No.(approx. avg. cell count): Obervations

1(1e6): Structure is broken up. A lot of loose, drifting cells. Cell density is lower than before, still a very dense outer ring at the edge of the well.

2(2e5): No significant changes: Continuous less dense structure with a reticular structure based on star like extensions of the cells. No distinct colonies. Same ring structure with high cell density.

3(4e4): Continuous structure with a reticular structure based on star like extensions of the cells, low cell density. No distinct colonies. Same ring structure with high cell density.

4(8e3): Regions with small cell clusters, also regions with bigger continuos structure and regions with lots of scattered individual cells. Clusters seem to have become less, but more distributed cells. Ability to distinct between colonies is diminished. More dense outer ring structure close to the edge.

5(1.6e3): No significant changes: Less individual cells than well no. 4. Lots of distinct cell clusters, most with <10 cells. Different degrees of compactness: some are very loose with multiple cell diameter space (<5 cell diameter) between them, some are closer and have connections and some are so close that individual cells are hard to identify. Varying sizes of clusters. Ability to distinct between colonies varies across the well, depending on amount of loose individual cells and closeness of neighboring colonies. Outer ring structure with high cell density.

6(320): No significant changes: Few, unevenly distributed cells. Only few clusters, with loose structure, and a few very compact ones. Also shows the ring structure now.

7(64): No significant changes: Very few individual cells.

8(12): No significant changes: Very few individual cells, no colonies found.

9(~2): No significant changes: No living cell found.

10(<1): No significant changes: No living cell found.