User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/30

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First observation on cell growth for dilution sequence

Well No.(approx. avg. cell count): Obervations

1(1e6): Continuos dense structure with a reticular structure based on star like extensions of the cells. No distinct colonies. Close to the edge of the well, but not exactly at the edge the cells form a very dense ring enclosing the whole plate.

2(2e5): Continuos less dense structure with a reticular structure based on star like extensions of the cells. No distinct colonies. Same ring structure with high cell density.

3(4e4): Unevenly distributed cells. Regions with close connections between cells and then wider gaps with few individual cells. Agglomerations cannot be described as distinct colonies. More dense ring structure close to the edge, these cells have a lot of connections, followed by an outer ring with only very few cells.

4(8e3): Regions with small yet distinct cell clusters, also regions with bigger continuos structure and regions with lots of scattered individual cells.

5(1.6e3): Less individual cells than well no. 4. Lots of distinct cell clusters, most with <10 cells. Different degrees of compactness: some are very loose with multiple cell diameter space between them, some are closer and have connections and some are so close that individual cells are hard to identify.

6(320): Few, unevenly distributed cells. Only few clusters, with loose structure.

7(64): Very few individual cells, found only one cluster with ~7 cells.

8(12): 3 different clusters found, very loose approx. 10 times cell diameter space between cells. All clusters are close to the edge.

9(~2): Only a handful individual cells found.

10(<1): Found only one dividing cell.