User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25

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Preparation of a dilution sequence

To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared. Cell line: HEK 293-Luc14 Plates: 6-well plates Dilution rate: 1:5 Starting with a full grown plate mixed into 10ml liquid.

Well No. : Approximate avg. cell count 1: 1,000,000 2: 200,000 3: 40,000 4: 8,000 5: 1,600 6: 320 7: 64 8: ~12 9: ~2 10:<1