Difference between revisions of "User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25"

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(Preparation of a dilution sequence)
(Preparation of a dilution sequence)
Line 8: Line 8:
 
==Preparation of a dilution sequence==
 
==Preparation of a dilution sequence==
 
To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.
 
To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.
 +
 
Cell line: HEK 293-Luc14
 
Cell line: HEK 293-Luc14
 +
 
Plates: 6-well plates
 
Plates: 6-well plates
 +
 
Dilution rate: 1:5
 
Dilution rate: 1:5
 +
 
Starting with a full grown plate mixed into 10ml liquid.
 
Starting with a full grown plate mixed into 10ml liquid.
  
 
Well No. : Approximate avg. cell count
 
Well No. : Approximate avg. cell count
 +
 
1:  1,000,000
 
1:  1,000,000
  
 
2:  200,000
 
2:  200,000
 +
 
3:  40,000
 
3:  40,000
 +
 
4:  8,000
 
4:  8,000
 +
 
5:  1,600
 
5:  1,600
 +
 
6:  320
 
6:  320
 +
 
7:  64
 
7:  64
 +
 
8:  ~12
 
8:  ~12
 +
 
9:  ~2
 
9:  ~2
 +
 
10:<1
 
10:<1
  

Revision as of 11:09, 30 January 2013

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Preparation of a dilution sequence

To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.

Cell line: HEK 293-Luc14

Plates: 6-well plates

Dilution rate: 1:5

Starting with a full grown plate mixed into 10ml liquid.

Well No. : Approximate avg. cell count

1: 1,000,000

2: 200,000

3: 40,000

4: 8,000

5: 1,600

6: 320

7: 64

8: ~12

9: ~2

10:<1