Difference between revisions of "User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25"

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(Autocreate 2013/01/25 Entry for User:David_Dreher/Notebook/Chromatin_controlled_cell_pattern)
 
(Preparation of a dilution sequence)
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==Entry title==
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==Preparation of a dilution sequence==
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To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.
  
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Cell line: HEK 293-Luc14
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Plates: 6-well plates
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Dilution rate: 1:5
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Starting with a full grown plate mixed into 10ml liquid.
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Well No. : Approximate avg. cell count
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1:  1,000,000
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2:  200,000
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3:  40,000
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4:  8,000
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5:  1,600
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6:  320
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7:  64
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8:  ~12
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9:  ~2
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10:<1
  
 
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Revision as of 10:09, 30 January 2013

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Preparation of a dilution sequence

To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.

Cell line: HEK 293-Luc14

Plates: 6-well plates

Dilution rate: 1:5

Starting with a full grown plate mixed into 10ml liquid.

Well No. : Approximate avg. cell count

1: 1,000,000

2: 200,000

3: 40,000

4: 8,000

5: 1,600

6: 320

7: 64

8: ~12

9: ~2

10:<1