User:David Dreher/Notebook/Chromatin controlled cell pattern/2013/01/25

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Revision as of 10:09, 30 January 2013 by David Dreher (talk | contribs) (Preparation of a dilution sequence)
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Preparation of a dilution sequence

To determine optimal cell densities for the cultures to grow into distinct cell colonies a dilution sequencen was prepared.

Cell line: HEK 293-Luc14

Plates: 6-well plates

Dilution rate: 1:5

Starting with a full grown plate mixed into 10ml liquid.

Well No. : Approximate avg. cell count

1: 1,000,000

2: 200,000

3: 40,000

4: 8,000

5: 1,600

6: 320

7: 64

8: ~12

9: ~2

10:<1