Difference between revisions of "User:Daniel Goodman/Notebook/Cluzel/2010/03/29"

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==Entry title==
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==Prepare for Afternoon/Evening==
* Insert content here...
 
  
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===Agarose===
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4% agarose,  4.5 ml, 0.18 mg low melt agarose
 +
heat at 80 degrees
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(put in heat bath at 1:00)
 +
===Cells===
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Put one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm)
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===Chips===
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Clean chips in hot water DI bath, 80 degrees
 +
(put in heat bath at 1:00)
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===Strains===
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Aliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight
  
 
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Latest revision as of 09:02, 29 March 2010

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Prepare for Afternoon/Evening

Agarose

4% agarose, 4.5 ml, 0.18 mg low melt agarose heat at 80 degrees (put in heat bath at 1:00)

Cells

Put one GFP colony from last week's plate in culture flash w/ 4ml LB, 4ul amp (shaker at 12:50pm)

Chips

Clean chips in hot water DI bath, 80 degrees (put in heat bath at 1:00)

Strains

Aliquot from Mark Kim's EZR-12-CFP strain, nanodrop, prepare cloning for tonight