Difference between revisions of "User:Daniel Goodman/Notebook/Cluzel/2010/03/05"

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==Try transferring again (w/ features)==
 
==Try transferring again (w/ features)==
 
*Scope access at 4PM
 
*Scope access at 4PM
*Start gel pouring at 2:30
+
*Start gel pouring at 2:30 - Poured 3% at 2:30, waiting for more melting for 5%
 
*Try w/ 3% and 5% agarose
 
*Try w/ 3% and 5% agarose
  

Revision as of 12:38, 5 March 2010

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Cluzel Lab Notebook
Daniel Goodman
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Grow cells

  • Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM)

Made agarose

  • Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM)

Make chip impression in solidified gel

  • Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down?

Results: no features found with significant 'push' on 3% gel.

Try transferring again (w/ features)

  • Scope access at 4PM
  • Start gel pouring at 2:30 - Poured 3% at 2:30, waiting for more melting for 5%
  • Try w/ 3% and 5% agarose

While waiting...

  • Play with a few featureless gels, practice flipping & transferring