Difference between revisions of "User:Daniel Goodman/Notebook/Cluzel/2010/03/05"

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==Entry title==
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==Grow cells==
* Insert content here...
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* Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM)
  
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==Made agarose==
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* Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM)
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==Make chip impression in solidified gel==
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* Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down?
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''Results: no features found with significant 'push' on 3% gel.''
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==Try transferring again (w/ features)==
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*Scope access at 4PM
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*Start gel pouring at 2:30
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*Try w/ 3% and 5% agarose
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==While waiting...==
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*Play with a few featureless gels, practice flipping & transferring
  
 
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Revision as of 11:05, 5 March 2010

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Cluzel Lab Notebook
Daniel Goodman
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Grow cells

  • Took a colony from fridge plate of MG1655 w/ GFP, put in 4 mL LB with 4 uL amp in 37°C shaker. (~10:30 AM)

Made agarose

  • Made 5 mL of 5% agarose, and 5 mL of 3% agarose. (~10:40 AM)

Make chip impression in solidified gel

  • Try quick experiment based on results from Wednesday; can we make features on a gel by pressing down?

Results: no features found with significant 'push' on 3% gel.

Try transferring again (w/ features)

  • Scope access at 4PM
  • Start gel pouring at 2:30
  • Try w/ 3% and 5% agarose

While waiting...

  • Play with a few featureless gels, practice flipping & transferring